Fig 4. cdipt mutants have significantly impaired motor function compared to their wildtype siblings.

A) Spontaneous swim movement was assessed by tracking 5-days or 6-days old zebrafish larvae over 1 hour. Representative examples of tracking plots of individual larvae movement. Black represents slow movement (<5 mm/s), green represents average speed (5–20 mm/s), and red represents fast movement (>20 mm/s). B) The cdipt mutant larvae are significantly slower than their WT siblings, both at 5dpf (WT n = 22, cdipt n = 26, p = 0.0318) and 6dpf (WT n = 36, cdipt n = 28, p = 0.0036). C) Involuntary motor function was assessed using an optovin-stimulated movement assay in response to pulses of light. Representative examples of tracking plots of individual larvae showing movement over 20 seconds, involving 5 seconds of white light exposure followed by 15 seconds of darkness. D) There is a significant difference between the average speed travelled by WT zebrafish compared to cdipt mutant zebrafish (n = 18 and 14, respectively; p < 0.0001, Student’s t test, 2-tailed). WT and cdipt mutant zebrafish plateau at the same rate (n = 7, p = 0.3487).