Fig 5. Localization of PIPs by immunofluorescence in wildtype and cdipt mutant zebrafish.

Confocal micrographs showing localization of PIPs is not affected in early larval development of cdipt mutants. (A, A’) visualization of skeletal muscle from live embryos injected with PLCδPH-GFP, a marker for PI(4,5)P₂. There was no obvious difference in expression between wild type (WT) and cdipt mutant embryos. (B-D, B’-D’) Immunostaining with PIP antibodies of myofibers isolated from WT and cdipt mutants. Localization of PI(4,5)P₂ (B, B’), PI3P (C, C’) and PI(3,4)P₂ (D, D’) is similar in wildtype and cdipt zebrafish. Scale bars = 10μm.