Table 1.
Examples of confined illumination strategies
| Light-sheet fluorescence microscopy | Detection numerical aperture | Example specimen type | Single-molecule localization microscopy | Reference |
|---|---|---|---|---|
| Multidirectional SPIM (mSPIM) | Not available | Zebrafish embryo | No | 49 |
| Airy beam | 0.4 | Juvenile amphioxus | No | 70 |
| Inverted SPIM (iSPIM) | 0.8 | C. elegans embryo | No | 44 |
| Dual-view iSPIM (DiSPIM) | 0.8 | C. elegans embryo, cell | No | 45 |
| Three-objective SPIM (TriSPIM) | 0.8 | Theoretical | No | 50 |
| Axially swept light-sheet microscopy (ASLM) | 0.8 | Cell | No | 144 |
| Tiling light-sheet SPIM (TLS-SPIM) | 0.8 | C. elegans embryo | No | 145 |
| 0.8 | C. elegans embryo | No | 146 | |
| Swept confocally aligned planar excitation (SCAPE) | 0.95 | Mouse, Drosophila larva | No | 147 |
| Oblique plane microscopy (OPM) | 1.35 | Pollen grain | No | 148 |
| Digital scanned laser light-sheet fluorescence microscopy (DLSM) | 0.075–1.0 | Zebrafish embryo | No | 149 |
| Selective plane illumination microscopy (SPIM) | 0.25–0.8 | Medaka embryo, Drosophila embryo | No | 43 |
| Light-sheet Bayesian microscopy (LSBM) | 1.0 | Cell | Yes | 56 |
| Individual molecule localization SPIM (IML-SPIM) | 0.8 and 1.1 | Spheroid | Yes | 46 |
| πSPIM | 1.1 | Cell | No | 51 |
| Lattice light-sheet microscopy (LLSM) | 1.1 | Cell, C. elegans embryo, Drosophila embryo | Yes | 47 |
| Bessel beam | 0.8 | Cell | No | 67 |
| 1.1 | Cell, C. elegans embryo and larva, Drosophila embryo and adult brain | No | 68 | |
| Lateral interference tilted excitation (LITE) | ≥1.2 | Cell, C. elegans embryo, Drosophila embryo | No | 150 |
| Single-objective light-sheet microscopy (SO-LSM) | 1.2 | Cell | Yes | 151 |
| Oblique plane stochastic optical reconstruction microscopy (obSTORM) | 1.2 | Cell, C. elegans, Drosophila larval brain, mouse retina and brain sections | Yes | 53 |
| Axial plane optical microscopy (APOM) | 1.4 | Pollen grain, mouse brain section | No | 74 |
| Epi-illumination SPIM (eSPIM) | 1.27 | Cell | Yes | 52 |
| Reflected light-sheet microscopy (RLSM) | 1.35 and 1.4 | Cell | Yes | 54 |
| Single-objective SPIM (soSPIM) | 0.3–1.4 | Cell, Drosophila embryo | Yes | 58 |
| Tilted light-sheet microscopy with 3-D PSFs (TILT3D) | 1.4 | Cell | Yes | 55 |
| Highly inclined swept tile (HIST) | 1.45 | Cell | No | 31 |
| Highly inclined and laminated optical sheet (HILO) | 1.49 | Cell | No | 57 |