Table 1. Effects of oligomycins and the fungicide Nativo® WG75 on germination of conidia and morphology of germ tubes and appressoria of Magnaporthe oryzae Triticum at 0.05 μg/ml in vitro.
Compound | Time (h) | Germination of conidia, and morphology of germ tubes and appressorial formation | |
---|---|---|---|
Germinated conidia (% ± SEa) | Morphological change/developmental transitions in the treated conidia | ||
Water | 0 | 0 ± 0e | No germination |
6 | 100 ± 0a | Germination with normal germ tube and normal appressoria | |
12 | 100 ± 0a | Normal mycelial growth | |
24 | 100 ± 0a | Normal mycelial growth | |
Oligomycin B | 0 | 0 ± 0e | No germination |
6 | 38.3 ± 0.7d | 24 ± 0.9% Short germ tube and 14.3 ± 0.7% conidia lysed | |
12 | 24 ± 0.9d | 10.6 ± 0.7% Normal germ tube, 8.4 ± 0.2% short and 5 ± 0.4% Abnormally elongated germ tube | |
24 | 0 ± 0b | No appressoria, no mycelial growth | |
Oligomycin F | 0 | 0 ± 0e | No germination |
6 | 59 ± 0.8b | 53 ± 0.4% Short germ tube and 6 ± 0.8% conidia lysed | |
12 | 53 ± 0.4b | 33.3 ± 0.5% Normal germ tube and 19.7 ± 0.2% abnormal branching at the tips | |
24 | 0 ± 0b | No appressoria, no mycelial growth | |
Nativo | 0 | 0 ± 0e | No germination |
6 | 50.3 ± 0.7c | Germinated with a short germ tube | |
12 | 50.3 ± 0.7c | Normal germ tube | |
24 | 0 ± 0b | No appressoria; no mycelial growth |
aThe data presented here are the mean value ± SE of three replicates in each compound. Means within the column followed by the same letter(s) are not significantly different from those assessed by Tukey's HSD (Honest Significance Difference) post-hoc (p ≤ 0.05). Conidia germination percent at different incubation times is not cumulative, rather at different time intervals.