Skip to main content
. 2020 Aug 17;15(8):e0233665. doi: 10.1371/journal.pone.0233665

Table 1. Effects of oligomycins and the fungicide Nativo® WG75 on germination of conidia and morphology of germ tubes and appressoria of Magnaporthe oryzae Triticum at 0.05 μg/ml in vitro.

Compound Time (h) Germination of conidia, and morphology of germ tubes and appressorial formation
Germinated conidia (% ± SEa) Morphological change/developmental transitions in the treated conidia
Water 0 0 ± 0e No germination
6 100 ± 0a Germination with normal germ tube and normal appressoria
12 100 ± 0a Normal mycelial growth
24 100 ± 0a Normal mycelial growth
Oligomycin B 0 0 ± 0e No germination
6 38.3 ± 0.7d 24 ± 0.9% Short germ tube and 14.3 ± 0.7% conidia lysed
12 24 ± 0.9d 10.6 ± 0.7% Normal germ tube, 8.4 ± 0.2% short and 5 ± 0.4% Abnormally elongated germ tube
24 0 ± 0b No appressoria, no mycelial growth
Oligomycin F 0 0 ± 0e No germination
6 59 ± 0.8b 53 ± 0.4% Short germ tube and 6 ± 0.8% conidia lysed
12 53 ± 0.4b 33.3 ± 0.5% Normal germ tube and 19.7 ± 0.2% abnormal branching at the tips
24 0 ± 0b No appressoria, no mycelial growth
Nativo 0 0 ± 0e No germination
6 50.3 ± 0.7c Germinated with a short germ tube
12 50.3 ± 0.7c Normal germ tube
24 0 ± 0b No appressoria; no mycelial growth

aThe data presented here are the mean value ± SE of three replicates in each compound. Means within the column followed by the same letter(s) are not significantly different from those assessed by Tukey's HSD (Honest Significance Difference) post-hoc (p ≤ 0.05). Conidia germination percent at different incubation times is not cumulative, rather at different time intervals.