Fig 3. Generation of a mouse model carrying a frameshift variant in Magel2.

(A) We obtained genome-edited mice carrying different variants in Magel2. We selected #1.10 as the founder mouse. (B) Comparison of the base sequence and amino acid residues in Magel2. Founder mouse #1.10 carried a homozygous frameshift variant in Magel2. (C) The pedigree of our mouse model. Mice carrying a variant in the paternal allele of Magel2 were termed ‘Magel2^P:fs’. Mice carrying a variant in the maternal allele of Magel2 were termed ‘Magel2 ^M:fs’. Littermates not carrying a variant in Magel2 were termed ‘Magel2⁺’.