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. 2020 Aug 17;15(8):e0231234. doi: 10.1371/journal.pone.0231234

Fig 5. Gene expression analysis of RNA extracted from left ventricle (LV) of 20-week-old obese and lean ZSF1 male rats.

Fig 5

The gene expression analysis of RNA revealed increased expression of 56 heart abundant genes and decreased expression of 48 heart abundant genes; the heatmap represents the individual expression changes in obese vs lean ZSF1 rats (A). Ingenuity pathway analysis (IPA) of enriched metabolic pathways (B) and comparison of differentially expressed genes (DEGs) presented in the enriched metabolic pathways from IPA over-represented cardiometabolic Medical Subject Headings (MeSH) terms (C). IPA of 267 significantly increased (FC > 1.5, Benjamini-Hochberg corrected p-value < 0.05) and 431 significantly decreased genes (FC < 2/3, p-value < 0.05) in LV of obese ZSF1 rats (D); comparison of DEGs enriched in cardiovascular disease (CVD), impaired glucose metabolism, and lipid metabolism error disease pathways (E); and comparison of DEGs associated with different subgroups of heart disease pathways from over-representative MeSH term analysis (F). Gene symbols in bold indicate increase of expression and in italic indicate decrease of expression. n = 8 for lean and n = 8 for obese ZSF1 groups.