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. 2020 Aug 5;13:7793–7805. doi: 10.2147/OTT.S249494

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© 2020 Yu et al.

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Knocking down of circ_0005576 inhibited proliferation and promoted apoptosis of CRC cells. (A) qRT-PCR was performed to detect the transfection efficiency. Results indicated that circ_0005576 expression in SW620 and SW480 cells was obviously regulated by transfection. (B) CCK-8 assay showed that knockdown of circ_0005576 prominently inhibited CRC cells proliferation. (C) According to EdU experiment, knockdown of circ_0005576 significantly reduced EdU positive cells (red fluorescence). This indicated that knockdown of circ_0005576 obviously inhibited CRC cells proliferation. (D) Flow cytometry analysis revealed that knockdown of circ_0005576 markedly increased cells in G1 phase and decreased cells in S phase. (E) Flow cytometry analysis indicated that knockdown of circ_0005576 remarkably induced apoptosis of CRC cells. **P < 0.01 and ***P < 0.001.

Abbreviations: NC, negative control; OD, optical density; DAPI, 4′-6-diamidino-2-phenylindole; EdU, 5-ethynyl-2-deoxy-uridine; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8.