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. 2020 Aug 5;13:7793–7805. doi: 10.2147/OTT.S249494

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© 2020 Yu et al.

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Circ_0005576 promoted CDK8 expression through suppressing miR-874 in CRC. (A) The binding site between circ_0005576 and miR-874. (B) Luciferase reporter assay and RNA pull-down assay confirmed that circ_0005576 acted as miR-874 sponge. (C) qRT-PCR showed that down-regulation of circ_0005576 significantly elevated miR-874 expression in SW620 and SW480 cells. (D) According to qRT-PCR, miR-874 expression was markedly declined in CRC tumor tissues than that in adjacent normal tissues. (E) Circ_0005576 and miR-874 in CRC tumor tissues exhibited a significant negative correlation. (F) CDK8 possessed binding site for miR-874. (G) Luciferase reporter assay verified that CDK8 was directly suppressed by miR-874. (H) qRT-PCR indicated that miR-874 up-regulation reduced the expression of CDK8 mRNA in SW620 and SW480 cells. (I) Western blot analysis revealed that miR-874 up-regulation diminished the expression of CDK8 protein in SW620 and SW480 cells. (J) CDK8 expression in CRC tumor tissues was dramatically increased when compared with normal tissues. (K) The CDK8 expression was negatively correlated with miR-874 expression in CRC tumor tissues. **P < 0.01 and ***P < 0.001.

Abbreviations: CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; NC, negative control; wt, wild type; mut, mutant type; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.