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. 2020 Aug 5;13:7793–7805. doi: 10.2147/OTT.S249494

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© 2020 Yu et al.

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miR-874 knockdown and CDK8 overexpression reversed the inhibitory effect of circ_0005576 knockdown on CRC development. (A) qRT-PCR was performed to detect the transfection efficiency. Data illustrated that CDK8 mRNA and protein expression in SW620 and SW480 cells was successfully regulated by transfection. (B) CCK-8 assay indicated that circ_0005576 knockdown inhibited SW620 and SW480 cell proliferation. However, miR-874 knockdown and CDK8 overexpression reversed this inhibitory effect. (C) EdU experiment revealed that circ_0005576 knockdown inhibited SW620 and SW480 cell proliferation. However, miR-874 knockdown and CDK8 overexpression reversed this inhibitory effect. (D) According to flow cytometry analysis, circ_0005576 knockdown arrested SW620 and SW480 cells in G1 phase and reduced cells in S phase. However, miR-874 knockdown and CDK8 overexpression reduced cells in G1 phase and elevated cells in S phase. (E) Flow cytometry analysis exhibited that circ_0005576 knockdown induced SW620 and SW480 cells apoptosis. However, miR-874 knockdown and CDK8 overexpression reduced apoptosis of SW620 and SW480 cell. *P < 0.05 or **P < 0.01.

Abbreviations: CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; OD, optical density; DAPI, 4′-6-diamidino-2-phenylindole; EdU, 5-ethynyl-2-deoxy-uridine; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.