Fig. 1.

Nanopore DNA sequencing and associated data analysis. A consumable flowcell containing an array of hundreds or thousands of such nanopores is loaded into the sequencing device (e.g. MinION). Ionic current (in pico amperes) is measured when DNA strands pass through nanopores to produce the raw signal, which is eventually basecalled. The base-called reads are then aligned to a reference genome. The raw signal is then revisited during the polishing step. Images of nanopore devices are reproduced with permission from ONT