Table 2.
Effect of ethanolic extract of Iris songarica (IS) on hepatic and renal MDA and SOD
| Groups |
Organ index
(g/g body weight)*100 |
MDA (nmol/mg protein)
|
SOD (U/mg protein)
|
|||
|---|---|---|---|---|---|---|
| Liver | Kidney | Liver | Kidney | Liver | Kidney | |
| Control | 2.56±0.15## | 0.38±0.02 | 1.98±0.107### | 1.69±0.09### | 2.96±0.13### | 1.87±0.12### |
| MTX | 3.13 ±0.16** | 0.35±0.04 | 3.11±0.18*** | 4.79±0.28*** | 1.66±0.12*** | 1.55±0.11*** |
| MTX+IS50 | 3.24±0.32 | 0.38±0.04 | 1.95±0.05###,&,† | 2.85±0.106###,†,& | 1.75±0.11***,### | 1.83±0.12***,### |
| MTX+IS150 | 3.40±0.17*** | 0.37±0.02 | 1.66±0.09***,###,@,† | 2.46±0.09***,###,@,& | 1.75±0.12***,### | 1.78±0.11***,### |
| MTX+IS300 | 3.33±0.26*** | 0.40±0.04 | 1.20±0.16***,###,&,† | 2.31±0.22***,###,@ | 1.75±0.11***,### | 1.69±0.11***,### |
Values are presented as mean±SD (n=8). Data were analyzed by one-way ANOVA test followed by Dunnett's test. *p<0.05, **p<0.01 and ***p<0.001, show significant difference compared to control group. # p<0.05, ## p<0.01, and ### p<0.001, show significant difference compared to MTX group respectively.@, † and & show significant difference at level of p<0.001 compared to IS50, IS150 and IS300 groups respectively. MTX: methotrexate (20 mg/kg), IS50-IS300: ethanolic extract of Iris songarica at the doses of 50-300 mg/kg. MDA: malondialdehyde, SOD: superoxide dismutase.