Skip to main content
. 2020 Jul 27;117(32):19566–19577. doi: 10.1073/pnas.2011228117

Fig. 2.

Fig. 2.

mGluR5 is required in female but not in male SF1 neurons for the regulation of glucose and lipid balance and sympathetic output. (A) Glucose tolerance test (GTT) in mGluR52L/2L:SF1-cre (n = 6) and mGluR52L/2L (n = 7) females. *Two-way ANOVA: genotype, P = 0.02; time, P < 0.0001; interaction, P = 0.006. Bonferroni’s multiple comparisons test: **P < 0.05. (B) Area under the curve (AUC) for the GTT in females. Two-tailed unpaired t test: *P = 0.04. (C) Glucose tolerance test (GTT) in mGluR52L/2L:SF1-cre (n = 6) and mGluR52L/2L (n = 6) males. (D) AUC for the GTT in males. (E) Insulin tolerance test (ITT) in mGluR52L/2L:SF1-cre (n = 10) and mGluR52L/2L (n = 11) females. *Two-way ANOVA: genotype, P = 0.02. Bonferroni’s multiple comparisons test: #P = 0.08; **P = 0.03. (F) AUC for the ITT in females. Two-tailed unpaired t test: *P = 0.02. (G) ITT in mGluR52L/2L:SF1-cre (n = 9) and mGluR52L/2L (n = 8) males. (H) AUC for the ITT in males. (I) Serum levels of insulin in fasted control (n = 11) and mutant (n = 12) females and control (n = 7) and mutant (n = 9) males. *Two-way ANOVA: genotype, P = 0.04; sex, P = 0.02. Bonferroni’s multiple comparisons test: **P = 0.04; ***P = 0.03. (J) Triglyceride (TG) levels in WAT of control and mutant females (n = 6) and control and mutant males (n = 5). *Two-way ANOVA: genotype, P = 0.02; interaction of sex and genotype, P = 0.03. Bonferroni’s multiple comparisons test: **P = 0.01. (K) Adipocyte cell area in mGluR52L/2L:SF1-cre and mGluR52L/2L (n = 5) females. Two-tailed unpaired t test: *P < 0.04. (L) Representative images of WAT in control and mutant females. (M) Serum levels of norepinephrine (NE) in control and mutant females (n = 8) and control (n = 6) and mutant males (n = 5). Two-tailed unpaired t test: *P < 0.01. Data are presented as means ± SEM.