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. 2020 Jul 27;117(32):19566–19577. doi: 10.1073/pnas.2011228117

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Fig. 5. — mGluR5 critically regulates the firing rate and intrinsic excitability of SF1 neurons in females, but not in males, and activity responses of these cells to estradiol. Representative traces of SF1 neurons at resting membrane potential from mGluR5^2L/2L and mGluR5^{2L/2L:SF1-cre} males (A) and naïve (B), OVX + vehicle (C), and OVX + E2 (D) mGluR5^2L/2L and mGluR5^{2L/2L:SF1-cre} females. (E) Spike frequency of SF1 neurons in mGluR5^2L/2L (n = 12 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 13 cells, n = 5 animals) males. (F) Spike frequency of SF1 neurons in naïve mGluR5^2L/2L (n = 12 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 14 cells, n = 4 animals) females, OVX mGluR5^2L/2L (n = 9 cells, n = 3 animals) and mGluR5^{2L/2L:SF1-cre} (n = 12 cells, n = 4 animals) females treated with vehicle, and OVX mGluR5^2L/2L (n = 10 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 9 cells, n = 3 animals) E2-treated females. *Two-way ANOVA: genotype, P = 0.005; interaction of genotype and treatment, P = 0.01. Bonferroni’s multiple comparisons test: **P = 0.02; ***P = 0.006; ****P = 0.002; ^#P = 0.1. (G) Neuronal firing rate (Hz) of SF1 neurons in response to increasing 20-pA current injection (pA) steps and in mGluR5^{2L/2L:SF1-cre} (n = 14 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 14 cells, n = 5 animals) males. (H) Resting membrane potential of SF1 neurons in mGluR5^{2L/2L:SF1-cre} (n = 14 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 14 cells, n = 5 animals) males. (I) Neuronal firing rate (Hz) of SF1 neurons in response to increasing 20-pA current injection (pA) steps and in mGluR5^{2L/2L:SF1-cre} (n = 13 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 15 cells, n = 4 animals) females. Two-way ANOVA: genotype, P < 0.0001. Bonferroni’s multiple comparisons test: *P < 0.05; **P < 0.01. (J) Resting membrane potential of SF1 neurons in mGluR5^{2L/2L:SF1-cre} (n = 13 cells, n = 4 animals) and mGluR5^{2L/2L:SF1-cre} (n = 15 cells, n = 4 animals) males. (K) c-fos⁺ cells in VMH (arrows) of fasted mGluR5^{2L/2L:SF1-cre} (n = 3) and mGluR5^{2L/2L:SF1-cre} (n = 4) females 30 min following injection of a bolus of glucose. *P = 0.01, unpaired t test. Data presented as means ± SEM.