Fig. 2.
RTP4 is a negative regulator of IFN-I response in vitro. (A and B) Luciferase signals (Luc signal) driven by IFN-β promoter after transfection of 2 × 105 293T cells with 100 ng empty plasmid vector (EV) or 100 ng plasmid encoding RTP4 after stimulation with poly(I:C) (500 ng) or poly(dA:dT) (125 ng). (C–I) Cotransfection of 2 × 105 293T cells with empty vector or plasmid (100 ng each) encoding the indicated proteins and RTP4. Luciferase signals were measured as in B. (J–M) Dose–responses of luciferase signals from 2 × 105 293T cells after cotransfection of the indicated genes with different amounts of plasmid (50, 100, and 200 ng) encoding RTP4. Mann–Whitney test, means and SD (n = 5 to 8); **P < 0.01; ns, not significant. All experiments were independently repeated with similar results.