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. 2020 Jul 27;117(32):19425–19434. doi: 10.1073/pnas.2003913117

Fig. 1.

Fig. 1.

CORIN promoter activities in uterine endometrial cells and cardiomyocytes. (A) Illustration of a luciferase construct with a 405-bp CORIN promoter fragment with an E-box–binding site, two KLF-binding sites, and a GATA-binding site (P405) and two constructs with 236- and 161-bp, respectively, truncated CORIN promoter fragments (P236 and P161). (B and C) Luciferase activities in endometrial AN3-CA (B) and HL-1 (C) cells transfected with the CORIN promoter constructs. The pGL3 construct was used as a background control. (D) Illustration of CORIN promoter constructs with mutations (boldface italic letters) in one (KM1 and KM2) or both (KM3) of the KLF-binding sites. (E and F) Luciferase activities in AN3-CA (E) and HL-1 (F) cells transfected with the CORIN promoter constructs. (G) Illustration of CORIN promoter construct with mutations (boldface italic letters) in the GATA-binding site (GM). (H and I) Luciferase activities in AN3-CA (H) and HL-1 (I) cells transfected with the CORIN promoter constructs. n = 3 to 6 per group. Data are presented as mean ± SEM; P values were analyzed by two-tailed Student’s t test. ns, not significant.