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. 2020 Jul 27;117(32):19425–19434. doi: 10.1073/pnas.2003913117

Fig. 3.

Fig. 3.

Effects of recombinant KLF protein expression on CORIN promoter activities in uterine endometrial AN3-CA cells. (A) Illustration of CORIN promoter constructs used. (B) Luciferase activities in AN3-CA cells cotransfected with the P236 CORIN promoter construct and a control vector (Vec) or plasmids expressing human KLF2 (K2), KLF9 (K9), KLF15 (K15), or KLF17 (K17). n = 8 per group. Data are presented as mean ± SEM; P values were analyzed by ANOVA. ns, not significant. (C) Western blotting of recombinant KLF proteins in the transfected cells using an anti-FLAG antibody. (D and E) Luciferase activities in AN3-CA cells transfected with the P405 CORIN promoter construct (positive control) or cotransfected with the P161 (D) or KM3 (E) CORIN promoter construct and a control vector (Vec) or plasmids expressing human KLF2 (K2), KLF9 (K9), KLF15 (K15), or KLF17 (K17). n = 3 per group. Data are presented as mean ± SEM; P values (vs. P405) and ns (not significant) (vs. vector-cotransfected group) were analyzed by ANOVA.