Fig. 4.

KLF17 and KLF2 expression in decidualized HESCs. (A) Cultured HESCs were induced to undergo decidualization. PRL (Top) and CORIN (Bottom) mRNA levels at indicated time points were analyzed by qRT-PCR. (B and C) Western blotting of endogenous KLF17 (B) and KLF2 (C) proteins in HESCs cultured with the decidualization medium over time. GAPDH was used in Western blotting (Top) to calculate relative protein expression levels (Bottom). n = 5 per group in A; n = 3 per group in B and C. In bar graphs, data are presented as mean ± SEM; ns, not significant; **P < 0.01 vs. the control at time 0 in the same experiment, as analyzed by ANOVA. (D and E) Immunohistochemistry (D) and coimmunofluorescent staining (E) of endogenous corin and KLF17 proteins in control (uninduced) and decidualized (induced) HESCs. In D, normal IgG was used as a negative control. Data are representative of three independent experiments. (Scale bars, 20 μm.)