Table 1.
Assay performance against the composite reference standard
|
Assay |
Target |
Performance characteristics |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
TP |
TN |
FP |
FN |
Sensitivity (95 % CI) |
Specificity (95 % CI) |
Accuracy (95 % CI) |
Misclassification rate (95 % CI) |
Kappa (95 % CI) |
||
|
FluA/B real-time RT-PCR |
FluA (total) |
151 |
960 |
0 |
0 |
100.0 (98.1–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
100.0 (97.8–100.0) |
|
FluA (H1N1) |
96 |
1015 |
0 |
0 |
100.0 (97.1–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
100.0 (96.8–100.0) |
|
|
FluA (H3N2) |
55 |
1056 |
0 |
0 |
100.0 (94.9–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
100.0 (94.7–100.0) |
|
|
FluB (Total) |
265 |
846 |
0 |
0 |
100.0 (98.9–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
100.0 (98.6–100.0) |
|
|
FluB (Yamagata) |
184 |
927 |
0 |
0 |
100.0 (98.5–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
100.0 (98.2–100.0) |
|
|
FluB (Victoria) |
81 |
1030 |
0 |
0 |
100.0 (96.5–100.0) |
100.0 (99.7–100.0) |
100.0 (99.5–100.0) |
0.0 (0.0–0.5) |
96.3 (96.3–100.0) |
|
|
RV15 |
FluA (total) |
148 |
960 |
0 |
3 |
98.0 (95.8–98.0) |
100.0 (99.6–100.0) |
99.7 (99.1–99.7) |
0.3 (0.3–0.9) |
98.8 (96.2–98.8) |
|
FluA (H1N1) |
94 |
1015 |
0 |
2 |
97.9 (94.9–97.9) |
100.0 (99.7–100.0) |
99.8 (99.2–99.8) |
0.2 (0.2–0.8) |
98.8 (95.1–98.8) |
|
|
FluA (H3N2) |
54 |
1056 |
0 |
1 |
98.2 (92.7–98.2) |
100.0 (99.7–100.0) |
99.9 (99.4–99.9) |
0.1 (0.1–0.6) |
99.0 (93.2–99.0) |
|
|
FluB (Total) |
263 |
846 |
0 |
2 |
99.2 (98.0–99.2) |
100.0 (99.6–100.0) |
99.8 (99.2–99.8) |
0.2 (0.2–0.8) |
99.5 (97.9–99.5) |
|
|
FluB (Yamagata) |
183 |
927 |
0 |
1 |
99.5 (97.8–99.5) |
100.0 (99.7–100.0) |
99.9 (99.4–99.9) |
0.1 (0.1–0.6) |
99.7 (97.7–99.7) |
|
|
FluB (Victoria) |
80 |
1030 |
0 |
1 |
98.8 (95.0–98.8) |
100.0 (99.7–100.0) |
99.9 (99.4–99.9) |
0.1 (0.2–0.6) |
99.3 (95.2–99.3) |
|
CIs, confidence intervals; FluA, influenza A; FluB, influenza B; FN, false negative; FP, false positive; TN, true negative; TP, true positive.