Table 1.
E. coli strains used in this study
Strain |
Genotype/serotype |
Syndrome* |
Spot test result† |
Plaque formation‡ |
Source§ |
---|---|---|---|---|---|
EN1E-0007 |
EAEC O86:H27 |
D |
+ |
+ |
[66] |
LRT9 |
EPEC O11:abH2 |
U |
+ |
+ |
[67] |
MC4100 |
F-araD139 Δ(argF-lac) U169 rpsL 150 StrR relA1 flhD5301 deoC1 ptsF25 rbsR |
NP |
+ |
+ |
[68] |
BC/Ae017.2 |
EAEC AMR, AMCR |
D |
− |
− |
UB|| |
BC/AaUIM099.2 |
EAEC |
D |
+ |
− |
UB |
BC/AaUIM171.3 |
EAEC AMR |
D |
− |
− |
UB |
BC/AaUIM206.1 |
EAEC |
D |
+ |
− |
UB |
BC/Ac277.1 |
EAEC |
D |
− |
− |
UB |
BC/Ac338.3 |
EAEC AMR, AMCR |
D |
+ |
+ |
UB |
BC/AaUIM363.2 |
EAEC |
D |
+ |
+ |
UB |
BC/Ac408.1 |
EAEC |
D |
+ |
− |
UB |
BC/Ac422.2 |
EAEC |
D |
− |
− |
UB |
BC/Ac482.1 |
EAEC AMR, SXTR |
D |
− |
− |
UB |
BC/AaUIM517.1 |
EAEC |
D |
− |
− |
UB |
BC/Ac512.2 |
EAEC |
D |
− |
+ |
UB |
*D, acute diarrhoea; U, details unknown; NP, non-pathogenic laboratory strain.
†For the spot test, phage in 5 µl drops were placed on streaks of the different clinical isolates, and then screened for clearing after overnight growth. A plus sign indicates that the strain was lysed by Escherichia virus PDX.
‡Plaque formation was assessed with serial dilutions of the PDX phage, propagated on different host strains using the pour plate method. In this case, individual plaques indicated reproductive infections [20]. A plus sign indicates PDX replicates (can form plaques on) the strain.
§Clinical isolate EN1E-0007 obtained from Tennessee, UB isolates obtained from Columbia
||Division of Pediatrics Infectious Diseases, University at Buffalo.