Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent Saccharomyces cerevisiae |
W303 background strains with different gene deletions | various | (See Materials and methods section) | |
| Recombinant DNA reagent | Yeast expression plasmids and recombination systems | various | (See Materials and methods section) | |
| Sequence-based reagent | Primers for DRIP and RT-PCR | Condalab | (See Materials and methods section) | |
| Antibody | Cy3 conjugated anti-mouse (goat monoclonal) | Jackson laboratories | Cat# 115-165-003, RRID:AB_2338680 | IF (1:1000) |
| Antibody | S9.6 anti DNA:RNA hybrids (mouse monoclonal) | ATCC Hybridoma cell line | Cat# HB-8730, RRID:CVCL_G144 |
DRIP (1 mg/ml) and IF (1:1000) |
| Commercial assay, kit | Macherey-Nagel DNA purification | Macherey- Nagel | Cat# 740588.250 |
|
| Commercial assay, kit | Qiagen’s RNeasy | Quiagen | Cat# 75162 | |
| Commercial assay, kit | Reverse Transcription kit | Qiagen | Cat # 205311 | |
| Peptide, recombinant protein | Zymolyase 20T | US Biological | Z1001 | (50 mg/ml) |
| Chemical compound, drug | Doxycyclin hyclate | Sigma-Aldrich | D9891 | (5 mg/ml) |
| Peptide, recombinant protein | Proteinase K (PCR grade) | Roche | Cat # 03508811103 | |
| Peptide, recombinant protein | Rnase A | Roche | Cat # 10154105103 | |
| Software, algorithm | GraphPad Prism V8.4.2 | GraphPad Software, La Jolla, CA, USA |
RRID:SCR_002798 | |
| Other | iTaq Universal SYBR Green | Bio-RAD | Cat # 1725120 | |
| Other | DAPI stain | Invitrogen | D1306 | 1 µg/mL |