Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Commercial assay or kit | KAPA Hyper Prep Kit | KAPA Biosystems | ||
| Commercial assay or kit | Vivaspin columns (MWCO 30 KDa) | GE healthcare | ||
| Peptide, recombinant protein | Xenopus H4 | Histone Source | ||
| Strain, strain background (C. elegans) | WT | Caenorhabditis Genetics Center | N2 | |
| Strain, strain background (C. elegans) | set-32 | PMID:30463021 | LG I: set-32(red11) | |
| Strain, strain background (C. elegans) | hrde-1 | Caenorhabditis Genetics Center | LG III: hrde-1(tm1200) | |
| Strain, strain background (C. elegans) | glp-1 | Caenorhabditis Genetics Center | glp-1(e2141) | |
| Strain, strain background (C. elegans) | met-2, set-25 | Caenorhabditis Genetics Center | met-2(n4256) set-25 (ok5021) | |
| Antibody | rabbit polyclonal anti-H3K9me3 | Abcam | ab8898 | Anitbody for ChIP seq |
| Antibody | rabbit polyclonal anti-H3K23me3 | Active Motif | 61500 | Anitbody for ChIP seq |
| Antibody | rabbit polyclonal anti-H3K23me2 | Active Motif | 39654 | Anitbody for ChIP seq |
| Antibody | rabbit polyclonal anti-H3K23me1 | Active Motif | 39388 | Anitbody for ChIP seq |
| Antibody | mouse monoclonal anti-H3K27me3 | Active Motif | 39535 | Anitbody for ChIP seq |
| Antibody | Donkey Anti-Rabbit-Alexa Fluor 488 | Jackson ImmunoResearch Laboratories | 711-545-152 | (1:300) |
| Antibody | monoclonal Mouse-anti-tubulin | DSHB | AA4.3 | (1:250) |
| Antibody | Cy5-conjugated donkey anti-Rabbit IgG secondary antibody | Jackson ImmunoResearch Laboratories | 711-175-152 | (1:1000) |
| Antibody | Cy5-conjugated donkey anti-Mouse IgG secondary antibody | Jackson ImmunoResearch Laboratories | 715-175-150 | (1:1000) |
| Peptide, recombinant protein | histone H3K23me3 | Active Motif | 31264 | antibody validation |
| Peptide, recombinant protein | histone H3K9me3 | Active Motif | 31601 | antibody validation |
| Peptide, recombinant protein | unmodified histone H3 | Abcam | ab2903 | antibody validation |
| Peptide, recombinant protein | H3K27me3 histone peptide | Abcam | ab1782 | antibody validation |
| Plasmid | pGEX-6p-1-GST-SET-32-WT | This study | pSG361 | recombinant protein expression |
| Plasmid | pGEX-6p-1-GST-SET-32-(Y448F) | This study | pSG434 | recombinant protein expression |
| Plasmid | pGEX-6p-1-GST-SET-25-WT | This study | pSG355 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_WT | Addgene, a gift from Joe Landry | 42631 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K4L | This study | pSG371 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K9L | This study | pSG367 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K14T | This study | pSG372 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K18L | This study | pSG373 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K23L | This study | pSG374 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K27L | This study | pSG375 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K36L | This study | pSG376 | recombinant protein expression |
| Plasmid | pet28a_human_H3.1_K37L | This study | pSG377 | recombinant protein expression |
| Plasmid | pet28a_elegans_H2A | This study | pSG395 | recombinant protein expression |
| Plasmid | pet28a_elegans_H2B | This study | pSG427 | recombinant protein expression |
| Plasmid | pet28a_elegans_H3.1 | This study | pSG428 | recombinant protein expression |
| Plasmid | L4440-oma-1 | PMID:28228846 | pSG42 | RNAi feeding plasmid against oma-1 |
| Plasmid | L4440-smg-1 | PMID:22231482 | pSG27 | RNAi feeding plasmid against smg-1 |
| Plasmid | L4440-GFP | A gift from Andrew Fire lab | L4417 | RNAi feeding plasmid against GFP |