Table 4. Accuracy of prediction of FluoroMyelin in human brain tissue slices across two developmental points (GW20 and GW24).

Median values of the Pearson correlation (r) and structural similarity index (SSIM) between predictions of image translation models and target fluorescence. We evaluated combinations of retardance (ρ), orientation x (ω_x), orientation y (ω_y), phase (Φ), and brightfield (BF) as inputs. These metrics are computed over 15% of the fields of view from two GW20 datasets and two GW24 test datasets. The 2D models take ∼ 4 hours to converge, whereas 2.5D models take ∼ 64 hours to converge.

Translation model	Input(s)	rxy	SSIMxy
Slice→Slice (2D)	BF	0.72	0.71
	ρ, Φ	0.82	0.82
	ρ, ωx, ωy, Φ	0.86	0.85
Stack→Slice (2.5D, $z=5$)	BF, ρ, ωx, ωy	0.87	0.85