Table 3.
Primer sets used for real-time quantitative reverse-transcription PCR
| Gene1 | Accession no. | Length, bp2 | Forward | Reverse |
|---|---|---|---|---|
| GYS-1 | NM_001195508.1 | 150 | CAGGACTGGAAGATTGGGAGG | AGTAGTTGTCGCCCCATTCA |
| INSR | XM_005654749.2 | 123 | GCCTTTCAAACGAGCAGGTG | GCATCTTGGGGTTGAACTGC |
| IRS-1 | NM_001244489.1 | 130 | AGAGGACCGTCAGTAGCTCA | GAAGGTGTGAGGTCCTGGTT |
| IGF1R | NM_214172.1 | 139 | GATTCAGGCCACCTCTCTCTCC | CCCTCCTACTATCAACAGAACGGC |
| IGF2 | NM_213883.2 | 212 | ACACCCTCCAGTTTGTCTGC | GGGGTATCTGGGGAAGTTGT |
| IGF2R | NM_001244473.1 | 194 | CAGGAACTGCTTTCTGAGCA | TGGAATCTGCCTTTTTCACC |
| LEPR | NM_001024587.1 | 82 | TCTGCTCCCCCAGAAAGGTA | CACAGGCACATGGCATTCAC |
| SREBP-1C | NM_214157.1 | 75 | AATAAATCCGCCGTCTTGCG | CTGCTTGAGCTTCTGGTTGC |
| LIPC | NM_001143714.1 | 119 | GCCTGGGATTAGAGCTACTGG | CTGACAGCCCTGATCGGTTT |
| PCK1 | NM_001123158.1 | 78 | CAAGGAGAGAAAACGTAGGCGA | TTTGAGAGCTGAGGAGGCAT |
| G6PC | NM_001113445.1 | 79 | TTGCTGGAGTCTTGTCAGGC | TTCTTGAGGCTGGCGTTGTA |
| PC | NM_214349.1 | 78 | TACGTCGCCCACAACTTCAG | GAAGCGCATCGCAACATCAA |
| FGF21 | NM_001163410.1 | 89 | CACGTCCCATTCCTGACTCC | AGTTTCCTGGGCATCATCCG |
| FBPase | NM_213979.1 | 130 | GAGTTCGACCCTGCCATCAC | TCCCTCCATAGACCAGCGTG |
| HPRT1 | XM_021079504.1 | 167 | GGGAGGCCATCACATCGTAG | CGCCCGTTGACTGGTCATTA |
| ACTB | XM_021086047 | 170 | AGATCAAGATCATCGCGCCT | ATGCAACTAACAGTCCGCCT |
| GAPDH | NM_001206359.1 | 84 | CATTGCCCTCAACGACCACT | ATGAGGTCCACCACCCTGTT |
| PPIA | NM_214353 | 174 | GCGTCTCCTTCGAGCTGTTT | ACTTGCCACCAGTGCCATTA |
1 GYS-1, glycogen synthase 1; INSR, insulin receptor; IRS-1, insulin receptor substrate 1; IGF1R, insulin like growth factor 1 receptor; IGF2, insulin like growth factor 2; IGF2R, insulin like growth factor 2 receptor; LEPR, leptin receptor; SREBP-1C, sterol regulatory element binding protein 1c; LIPC, hepatic lipase; PCK1, phosphoenolpyruvate carboxykinase 1; G6PC, glucose 6 phosphatase; PC, pyruvate carboxylase; FGF21, fibroblast growth factor 21; FBPase, fructose 1,6 bisphosphatase; HPRT1, hypoxanthine phosphoribosyltransferase 1.
2Amplicon length in base pairs.