Figure 1.
Characterization of tamoxifen-resistant breast cancer cell lines. (A) CCK-8 analysis of cell viability and IC50 values in parent and resistant cells treated with different concentrations of tamoxifen. (B) Western blot analysis of the level of ERα, vimentin, and E-cad in parent and resistant cells incubated with 10 μM tamoxifen. (C, D) Transwell analysis of the migration and invasion of parental and resistant cells treated with 10 μM tamoxifen. * P<0.05, ** P<0.01. CCK-8 – Cell Counting Kit-8; ERα – estrogen receptor alpha.