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. 2020 Aug 17;10:13913. doi: 10.1038/s41598-020-70383-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative analysis of the shared SNO-proteins in the juvenile (J) and adult (A) cortex. (a) Volcano Plot analysis was conducted on the shared SNO proteins in the J cortex vs. A cortex. The fold change (FC) was calculated as the difference of the relative abundance between A and J divided to J [FC = $\frac{Relative abundnce (A) - Relative abundance (J)}{relative abundance (J)}$ ] and normalized using log2. The horizontal line represents a significance level of P value= 0.05. The vertical lines represent the threshold of the FC = 1.3. The upregulated proteins are those that appear on the right side of the plot. These proteins meet the statistical significance criteria of both P < 0.05 and a FC > 1.3. (b) Biological processes of the significant up-regulated SNO-proteins. (c) Heat map analysis representing the differential relative abundance of the shared SNO-proteins in the J and A cortex. The abundance scale was normalized by – log10. Prism-GraphPad 8 was used to generate this figure.