Table 2.
Monobody MS3-6 and MS3-N3 interactome.
| Bait monobody | Cell line | STAT family proteins identifieda | Spectrum counts of razor and unique peptidesb | iBAQ (intensities)c | Rank out of all identified proteinsd |
|---|---|---|---|---|---|
| MS3-6 | |||||
| Jurkat | |||||
| Monobody | 498 | 6.74 × 109 | 2/578 | ||
| STAT3-FL | 599 | 1.23 × 109 | 6/578 | ||
| STAT3 Del-701 | 7 | 1.22 × 109 | 7/578 | ||
| STAT3-β | 3 | 1.21 × 109 | 8/578 | ||
| STAT1 | 2 | 4.52 × 105 | 425/578 | ||
| K562 | |||||
| Monobody | 1967 | 1.38 × 1010 | 1/566 | ||
| STAT3 [a.a. 3–84] | 5 | 5.05 × 109 | 3/566 | ||
| STAT3-FL | 3318 | 3.11 × 109 | 4/566 | ||
| STAT3-β | 14 | 3.11 × 109 | 5/566 | ||
| STAT3 Del-701 | 29 | 2.99 × 109 | 6/566 | ||
| STAT1 | 7 | 2.56 × 105 | 253/566 | ||
| STAT5B | 5 | 1.55 × 105 | 321/566 | ||
| MS3-N3 | |||||
| Jurkat | |||||
| Monobody | 62 | 8,43 × 109 | 3/399 | ||
| STAT3-FL | 173 | 4.63 × 107 | 41/399 | ||
| STAT3 Del-701 | 4 | 4.60 × 107 | 42/399 | ||
| STAT5B | 27 | 6.55 × 106 | 119/399 | ||
| STAT3-β | 1 | 5.98 × 106 | 120/399 | ||
| K562 | |||||
| Monobody | 517 | 1.91 × 1010 | 2/578 | ||
| STAT3-FL | 142 | 1.31 × 107 | 39/578 | ||
| STAT3-β | 2 | 1.11 × 107 | 41/578 | ||
| STAT3 Del-701 | 1 | 1.64 × 106 | 123/578 | ||
Exclusive spectrum counts of monobodies MS3-6 and MS3-N3 and STAT family members upon tandem affinity purification in Jurkat and K562 cells. The complete list of all identified proteins, including keratin contaminants and highly endogenously expressed proteins, is available as a PRIDE database submission: PXD018374.
aIncluding STAT3 isoforms.
bAll shared peptides between STAT family members and isoforms are associated to STAT3-FL, in addition to its unique peptides. All other STAT family members and isoforms display their unique peptides counts only.
ciBAQ values result from the sum of intensities of all tryptic peptides divided by the number of all theoretically observable peptides for each protein. These values provide an accurate determination of the relative abundance of all STATs identified in the samples.
dAll identified proteins, including common contaminants, such as keratin, were sorted by their iBAQ values from highest to lowest.