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. 2020 Aug 17;11:4116. doi: 10.1038/s41467-020-17885-z

Fig. 4. The ligand OSM regulates mitochondrial OXPHOS.

Fig. 4

a, b Bioenergetic analysis using a Seahorse XFe96 Bioenergetic Flux Analyzer in the absence and presence of OSM was performed in EGFRvIII-expressing BTSCs. BTSC73 (a): ***p < 0.0001 for each pairwise comparison except: **pBasal = 0.0045, ***pLeak = 0.0006; Unpaired two-tailed t-test, n = 6; BTSC147 (b): ***p < 0.0001 for each pairwise comparison; Unpaired two-tailed t-test, n ≥ 5. c, d Bioenergetic analysis in the absence and presence of OSM was performed in BTSCs lacking the EGFRvIII mutation. BTSC12 (c): ***p < 0.0001 for each pairwise comparison except: *pSRC = 0.0124; Unpaired two-tailed t-test, n = 6; BTSC145 (d): *pBasal = 0.0179, *pSRC = 0.0435, **pLeak = 0.0056, ***pMaximal = 0.0008; Unpaired two-tailed t-test, n = 4. e–g BTSC73 were subjected to bioenergetic analysis in the absence and presence of 10 ng/mL of OSM and either of pharmacological inhibitors 10 µM PD0325901, 10 µM LY294002, or 20 µM WP1066. PD0325901 (e): *pMaximal = 0.0303, **pATP-Linked = 0.0088, **pBasal = 0.0071; Unpaired two-tailed t-test, n = 6; LY294002 (f): *pATP-Linked = 0.0322, *pBasal = 0.0104, *pMaximal = 0.0101; Unpaired two-tailed t-test, n ≥ 6; WP1066 (g): **pATP-Linked = 0.0029, ***pBasal = 0.0001, ***pMaximal = 0.0002; Unpaired two-tailed t-test n ≥ 5. h OSMR-overexpressing primary CGN cultures were subjected to bioenergetic analysis in the absence or presence of OSM. **pLeak = 0.0014, ***pMaximal = 0.0001, ***pSRC < 0.0001; Unpaired two-tailed t-test, n ≥ 5. Data are presented as the mean ± SEM. n represents an independent biological sample.