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. 2020 Aug 11;8:713. doi: 10.3389/fcell.2020.00713

FIGURE 4.

FIGURE 4

Nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) deficiency did not influence activation and proliferation of cardiac fibroblasts (CFs), but coculture CFs with the supernatant of neonatal rat ventricular myocytes enhanced the activation and proliferation of it in vitro. (A) Immunohistochemical staining of α-smooth muscle actin and proliferating cell nuclear antigen in CFs of wild-type (WT) and knockout mice stimulated by isoproterenol (ISO) (n = 6). (B) Real-time PCR analyses of fibrotic markers (collagen I, collagen III, connective tissue growth factor, and Ki67) in each group (n = 6). *P < 0.05 vs. WT-phosphate-buffered saline group; #P < 0.05 vs. WT-ISO group. (C) Immunohistochemical staining of α-smooth muscle actin and proliferating cell nuclear antigen in CFs cocultured with the supernatant of neonatal rat ventricular myocytes treated with ISO and siNLRP3 or siNC (n = 6). (D) Real-time PCR analyses of fibrotic markers (collagen I, collagen III, and connective tissue growth factor) and Ki67in the indicated group. *P < 0.05 vs. knockout CFs + si NC-ISO supernatant; #P < 0.05 vs. WT CFs + si NC-ISO supernatant.