FIGURE 9.

Nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) regulated the production of ROS in neonatal rat ventricular myocytes (NRVMs). (A) ROS production of NRVMs transfected with si NLRP3 or si NC and stimulated by ISO or phosphate-buffered saline (PBS) (n = 6). (B–E) Protein expression of NOX2, NOX4, and SOD2 in NRVMs in the indicated group. Western blot images (B) and quantitative results (C–E) (n = 6). *P < 0.05 vs. PBS + siNC, ^#P < 0.05 vs. ISO + siNC. (F) ROS production of NRVMs transfected with Ad-NLRP3 or Ad-NC and stimulated by ISO or PBS (n = 6). (G–J) Protein expression of NOX2, NOX4, and SOD2 in NRVMs in the indicated group. Western blot images (G) and quantitative results (H–J) (n = 6). *P < 0.05 vs. PBS + Ad-NC, ^#P < 0.05 vs. ISO + Ad-NC.