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. 2020 Aug 11;12:230. doi: 10.3389/fnagi.2020.00230

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Copyright © 2020 Calabrese, Di Maio, Marino, Cardinale, Natale, De Rosa, Campanelli, Mancini, Napolitano, Avallone, Calabresi, Usiello, Ghiglieri and Picconi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–I) Western blot analysis of indicated proteins from contralateral and ipsilateral striata of 6-OHDA-lesioned rats treated with vehicle or rapamycin. (A,B) p-Ser845-GluA1 and p-Thr202/Tyr204-ERK42 protein levels in dopamine (DA) denerveted rats treated with vehicle (n = 5) or rapamycin (n = 4). (C,D) p-Ser240/244-S6 and TH protein levels (n = 5/treatment). (E,F) Protein expression of the subunits GluA1 and GluA2/3 of AMPA-type glutamate receptors in vehicle (n = 5) and rapamycin (n = 4) treated rats lesioned with 6-OHDA. (G,H) GluN1 and GluN2A NMDA receptor subunit levels in 6-OHDA vehicle or 6-OHDA rapamycin-treated rats (n = 5/treatment). (I) Expression of the GluN2B subunit in vehicle (n = 5) or rapamycin (n = 4) treated rats lesioned with 6-OHDA. (J–R) Western blot analysis of indicated proteins from contralateral and ipsilateral striata of sham-operated rats treated with vehicle or rapamycin (n = from 3 to 5/treatment). (J–L) Phosphorylation levels of GluA1 at Ser845, ERK42 at Thr202/Tyr204, and S6 at Ser240/244. (M) Tyrosine hydroxylase protein levels in contralateral and ipsilateral striata from sham-operated group. Protein expression of (N,O) AMPA (GluA1 and GluA2/3) and (P–R) NMDA glutamate receptor subunits (GluN1, GluN2A, and GluN2B). All data are expressed as mean ± SEM. Statistical significance was determined by two-way ANOVA, followed by Bonferroni multiple-comparisons test, ^##p < 0.01 for TH protein levels. For phospho-S6 levels (panels C,L), two-way ANOVA returned a significant treatment effect (^$$p < 0.001 and ^$p < 0.05 for panels C,L, respectively).