Fig. 2.

Expression of 3CprME and 3prME polypeptides in silkworm larvae (a), pupae (b), and Bm5 cells (c). In the case of silkworm larvae, hemolymph, and fat body were collected after infection with recombinant BmNPV. Homogenates of each sample were prepared according to the protocol described in “Materials and methods”. The 3CprME and 3prME polypeptides were detected by western blot using rat anti-PA tag as a primary antibody. d Hemolymph and fat body from silkworm larvae were verified for cross-reaction using mouse anti-E antibody (anti-E DENV-2) and mouse anti-prM antibody (anti-prM DENV-2) of DENV serotype 2