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. 2020 Aug 17;10:147. doi: 10.1186/s13568-020-01087-3

Table 1.

Used primers

Name 5′-3′
3CprME-F TAA TGG ATC CAT GAA TAA CCA GCG CAA GAA
3CprME-R-EcoRI TAA TGA ATT CTC AGA CTA CGT CGT CTT CCG C
3prME-F TAA TGG ATC CAT GTT TCA TCT CAC TTC CCG TGA TGG C
3prME-R-EcoRI TAA TGA ATT CTC AGA CTA CGT CGT CTT CCG CAC
pFastBac1-F TAT TCC GGA TTA TTC ATA CC
pFastBac1-R ACA AAT GTG GTA TGG CTG ATT
pUC/M13-F CCC AGT CAC GAC GTT GTA AAA CG
pUC/M13-R AGC GGA TAA CAA TTT CAC ACA GG

Underlines indicate restriction enzyme cleavage sites