Fig. 3.

Multiplication of shoots originating from somatic embryos of embryogenic line 27 under thidiazuron (TDZ) treatment in Pinus massoniana. Sprouting axillary buds ≥ 5 mm were counted for the calculation of total bud number per explant, and elongated buds ≥ 30 mm were used to calculate the number of effective buds per explant. Small letters indicate the differences among TDZ treatments for the number of total buds or effective buds per explant (Duncan’s tests, P ≤ 0.05), and capital letters indicate the differences between the number of total and effective buds within a TDZ treatment (t test, P ≤ 0.05)