Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 17;3:450. doi: 10.1038/s42003-020-01165-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Extracellular flux analysis of GSC-H cells treated for 12 h with the indicated concentrations of doranidazole under normoxic (a) or hypoxic (b) conditions. Representative examples of changes in OCR after sequential injection of the indicated inhibitors (Oligo oligomycin, Rtn rotenone, AA antimycin A) as well as quantitative data for basal OCR and spare respiratory capacity (means ± s.d. for n = 3 independent experiments) are shown. Mitochondrial complex I-, complex II-, and complex IV-dependent OCR (c) and complex II-, complex III-, and complex IV-dependent OCR (d) measured in GSC-H cells after exposure to the indicated concentrations of doranidazole for 12 h and permeabilization of the plasma membrane. Suc succinate, Malo malonate, Duro duroquinol. Data are means ± s.d. for at least five biologically distinct replicates in n = 1 experiment. e Immunoblot analysis of mitochondrial complex (C) proteins in GSC-H cells treated for 24 h with the indicated concentrations of doranidazole. f ATP content of GSC-H cells exposed to doranidazole under normoxic or hypoxic conditions for 12 h. Data are expressed relative to the value for normoxia and 0 mM doranidazole and are means ± s.d. from n = 3 three independent experiments. Statistical analysis was performed by one-way ANOVA followed by Dunnett’s post hoc test a, b, or with the two-way ANOVA followed by Sidak’s post hoc test f.