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. 2020 Aug 17;3:450. doi: 10.1038/s42003-020-01165-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Metabolome analysis for the TCA cycle in GSC-H cells exposed to 0 or 3 mM doranidazole for 24 h. N.D. not detected. The amounts of NAD⁺ and NADH and the NADH/NAD⁺ ratio (b) as well as ATP and AMP (c) and the total adenylate charge (d) were also determined. Metabolite levels were normalized by total protein amount, are means ± s.d. from eight biologically distinct replicates, n = 1 experiment, and were analyzed by the unpaired two-tailed Student’s t-test.