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. 2020 Aug 17;11(8):640. doi: 10.1038/s41419-020-02819-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b FXR expression in FXR-knockdown HT-29 and Caco-2 cells (a) or FXR-overexpressing SW480 and HCT116 cells (b) detected by western blotting analysis. c, d The effect of FXR knockdown (c) or overexpression (d) on the viability of colon cancer cells detected by CCK8 assays. e, f The effect of FXR knockdown (e) or overexpression (f) on colony formation of colon cancer cells. g, h The effect of FXR knockdown (g) or overexpression (h) on cell cycle distribution of colon cancer cells. All data are the mean ± SD of three independent experiments. *P < 0.05.