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. 2020 Aug 13;13:7997–8008. doi: 10.2147/OTT.S258616

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© 2020 Pu et al.

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PLK4 is a direct target gene of miR-654-3p. (A) The predicted miR-654-3p-binding site in the 3ʹUTR of PLK4 mRNA and its mutated version. (B–C) PLK4 mRNA and protein expression levels were measured by qRT-PCR and Western blot analysis using GAPDH as the loading control after transfection of the miR-654-3p inhibitor or mimic in A549 cells, respectively. (D) Dual-luciferase reporter assay. The relative luciferase activity was assessed after cells were co-transfected with the miR-654-3p mimic. The data are presented as the means ± SD of three measurements. **P < 0.01 vs the NC.