TABLE 1.

Cleaning and disinfection protocols for open surfaces and closed circuits.

ZONE	Open Plant Cleaning (OPC) of the surfaces							Cleaning In Place (CIP) of closed circuits and tanks
Period (wk)	1–3	4–5	6–7	8–9	10–11	12–13	14–15	1–15		4
Protocol	Conventional cleaning	Enzymatic cleaning				Conventional cleaning 2	Enzymatic cleaning
		Reinforced enzymatic cleaning	Routine enzymatic cleaning				Reinforced enzymatic cleaning 2	Conventional treatment		Reinforced enzymatic cleaning
Frequency	3x/wk (every 48 h)	3x/wk (every 48 h)	3x/wk (every 48 h)	2x/wk with enzymatic cleaning + 1x/wk with conventional cleaning	1x/wk with enzymatic cleaning + 2x/wk with conventional cleaning	3x/wk (every 48 h)	3x/wk (every 48 h)	3x/wk (every 48 h)	1x/wk (before the production week)	In addition to the conventional treatment
Cleaning	Alkaline chlorinated15 min, 40°C	Alkaline chlorinated15 min, 40°C	Alkaline chlorinated15 min, 40°C			Alkaline chlorinated15 min, 40°C	Alkaline chlorinated15 min, 40°C”	Caustic soda90 min, 85°C	Acid agent15 min, 85°C
	Rinsing	Rinsing	Rinsing			Rinsing	Rinsing	Rinsing	Rinsing
	Acid agent15 min,40°C	Foaming enzymatic detergent 3% (protease, lipase, amylase, oxydo reductase) 30 min, 40°C	Foaming enzymatic detergent 3% (protease, lipase, amylase)15 min, 40°C			Acid agent15 min, 40°C	Foaming enzymatic detergent 3% (protease, lipase, amylase, oxydo reductase) 30 min, 40°C	Alkaline chlorinated30 min, 85°C		Enzymatic detergent (protease, lipase, amylase, oxydo reductase)pH 7, 60 min, 45°CpH 9, 60 min, 45°C
	Rinsing	Rinsing	Rinsing			Rinsing	Rinsing	Rinsing
Disinfection	Quaternary ammonium15 min, 40°C					Quaternary ammonium15 min, 40°C
	Rinsing					Rinsing
	Peracetic acid15 min, 20°C	Peracetic acid15 min, 20°C	Peracetic acid15 min, 20°C			Peracetic acid15 min, 20°C	Peracetic acid15 min, 20°C	Peracetic acid30 min, 20°C		Peracetic acid30 min, 20°C
	Rinsing	Rinsing	Rinsing			Rinsing	Rinsing	Rinsing		Rinsing