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. 2020 Aug 11;11:484. doi: 10.3389/fendo.2020.00484

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Copyright © 2020 Biguetti, Couto, Silva, Shindo, Rosa, Shinohara, Andreo, Duarte, Wang, Brotto and Matsumoto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological characterization of bone healing 7 and 14 days post-surgery: Male and female, young (3 months old), and aged (18 months old) 129/SvEv WT and 5LOKO mice underwent our surgical injury model, and bone specimens were evaluated at 7 and 14 days post-surgery. (A) Representative images from young and aged animals (WT and 5LOKO) stained with H&E and Goldner's Trichrome/Alcian Blue. Images were captured at 10× magnification. (B) H&E representative images from aged animals at 14 days were captured at 100× magnification. Newly formed bone (NB), Osteoclast (blue arrows), Osteoblast (arrowhead), Blood Vessel (BV).