Figure 6.

Histomorphometric characterization and Immunolabeling of MuRF1 and MyoD in WT and 5LOKO mice: (A) Representative images for MuRF1 immunostaining and MyoD immunostaining (arrows) in male young SvEv WT mice at 7 days post-surgery. DAB was used for the antigen–antibody reaction. Muscle samples were left without counterstaining to be used for optical density quantification. (A–A′) Positive Pixel quantification was performed by the software Aperio Image Scope v/ 12.3.3 (Leica Biosystems, Buffalo Grove, USA). Images were analyzed with algorithm Positive Pixel Count version 9 and are expressed as Positivity (number of positive pixels/number total). Negative controls (NC) were used for calibration and confirmation. (B,C) Results from positive pixel quantification for MuRF1 and MyoD were presented as means ± SD for each marker. Different letters indicate significant differences between young (3 months old) and aged (18 months old) mice for each sex and strain (p < 0.05).