TABLE 2.

An overview of summary statistic for RRBS libraries constructed based on Ovation RRBS Methyl-Seq and our previously optimized method (gel-free method).

RRBS protocol	Adapter contamination	Read loss after trimming	Reads length (bp) distribution after trimming
Ovation method	Detected only in forward reads	51%	< 50   34%
			50–99  40%
			100–150   26%
Gel-free method	Detected in both reads	8%	< 50   4%
			50–99  29%
			100–150   67%

Paired-end reads from both methods were quality checked using fastQC software and trimming for Ovation libraries was performed according to manufacturer’s instructions. Read loss percentage was calculated as number of reads after trimming/number of reads before trimming. Bp, base pair.