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. 2020 Aug 11;11:1532. doi: 10.3389/fmicb.2020.01532

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Copyright © 2020 Liu, Wu, Mao, Guo, Zeng, Fei, Wan, Peng and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CRAB biofilm was removed effectively with Cec4. Confocal microscopy of CRAB biofilm without treatment (A) and after treatment with Cec4 (B). The three-dimensional reconstruction of CRAB biofilm without treatment (C) and after treatment with Cec4 (D). The total biomass volume (E) and thickness (F) of biofilm were calculated based on the fluorescence intensity. The results were averaged from three randomly selected positions of each sample. Data are expressed as mean ± standard deviation (SD). *p < 0.05; **p < 0.01.