FIGURE 2.

(A) Amide I region of the infrared spectra of TMD_TraJCD_TrwB, MobB_CloDF13, and MobBΔTMD. Proteins were purified, dialyzed against the corresponding buffer in D₂O and analyzed by IR spectroscopy as explained in “Materials and Methods” section. Obtained spectra were curve-fitted to show the different secondary structure components as detailed in Table 5. (B) Thermal denaturation of TMD_TraJCD_TrwB, MobB_CloDF13, and MobBΔTMD as seen by IR spectroscopy. The widths at half-height (WHH) of the amide I bands are plotted as a function of temperature (°C). Thermal denaturation is marked by an abrupt increase in bandwidth. Mid-point denaturation temperature (Tm) values corresponding to each protein are detailed in Table 5.