TABLE 1.
Plasmids employed in this work.
| Plasmid | Description | Phenotype | References |
| pET24a (+) | Expression vector | KanR, C-terminal His tag | Novagen |
| R388 | Natural plasmid | TmpR, TRAW, IncW | Datta and Hedges, 1972 |
| pKM101 | Natural plasmid | AmpR, TRAN, IncN | Langer and Walker, 1981 |
| pKM101ΔtraJ | pKM101:ΔtraJ | AmpR, TRAN, IncN, TraJ– | This work |
| pKM101Spcr_ΔtraJ | pKM101:ΔtraJ and SptR resistance cassette | SptR, TRAN, IncN, TraJ– | Whitaker et al., 2016 |
| pOPINE | Expression vector | AmpR, C-terminal His tag | Berrow et al., 2007 |
| pOPINE-3C-eGFP | Expression vector | AmpR | OPPF-UK (Addgene) |
| pOPINE-3C-eGFP-mobB | pOPINE-3C-eGFP:mobB | pOPINE-3C-eGFP:MobBCloDF13GFP expression under T7 promoter | This work |
| pOPINE-3C-eGFP-MobBΔTMD | pOPINE-3C-eGFP:MobBΔTMD | pOPINE-3C-eGFP:MobBΔTMDGFP expression under T7 promoter | This work |
| pOPINE-mobB | pOPINE:mobB | pOPINE:MobBCloDF13 expression under T7 promoter | This work |
| pOPINE-MobBΔTMD | pOPINE:MobBΔTMD | pOPINE:MobBΔTMD expression under T7 promoter | This work |
| pSU1456 | R388:ΔtrwB | SuR, TmpR, TRAW, IncW, TrwB– | Llosa et al., 1994 |
| pSU4814 | pSU19:mobCloDF13 | Chlr, Rep (p15A), MOB (CloDF13) | Nuñez and de la Cruz, 2001 |
| pSU4833 | pSU4814:ΔmobB | ChlR, Rep (p15A), MobB– | Nuñez and de la Cruz, 2001 |
| pUB9 | pWaldo-GFPe:trwB-GFP | KanR, TrwB-TEV-GFP-H8, expression under T7 promoter | Segura et al., 2014 |
| pUBQ4 | pET24a (+):traJTMD-trwBCD | KanR, TMDTraJCDTrwB-H6, expression under T7 promoter | This work |
| pUBQ4 (K142T) | pET24a (+):traJTMD-trwBCD (K142T) | KanR, TMDTraJCDTrwB-H6 containing the K142T mutation in the CDTrwB domain, expression under T7 promoter | This work |
| pUBQ4GFP | pWaldo-GFPe:traJTMD-trwBCD-GFP | KanR, TMDTraJCDTrwB-TEV-GFP-H8, expression under T7 promoter | This work |
| pWaldo-GFPe | Expression vector | KanR, C-Terminal GFP and His tag | Waldo et al., 1999 |