TABLE 2.
Oligonucleotides used in this work.
| Construct | Template | Protein | Sequence (5′ → 3′) | Cloning technology | |
| pOPINE-mobB | pSU4814 | MobBCloDF13 | F: | AGGAGATATACCATGTTTAATACGGATTCGCTTGCCTGGCAGTGG | In-fusiona |
| R: | GTGATGGTGATGTTTGTACAGCCCCGCAAAATCATCATCACCCG | ||||
| pOPINE-MobBΔTMD | pSU4814 | MobBΔTMD | F: | AGGAGATATACCATGGACGAGGCGGTGAACGCGAAAC | In-fusiona |
| R: | GTGATGGTGATGTTTGTACAGCCCCGCAAAATCATCATCAC | ||||
| pOPINE-3C-eGFP-mobB | pSU4814 | MobBCloDF13GFP | F: | AGGAGATATACCATGTTTAATACGGATTCGCTTGCCTGGCAGTGG | In-fusiona |
| R: | CAGAACTTCCAGTTTGTACAGCCCCGCAAAATCATCATCACCCG | ||||
| pOPINE-3C-eGFP-MobBΔTMD | pSU4814 | MobBΔTMDGFP | F: | AGGAGATATACCATGGACGAGGCGGTGAACGCGAAAC | In-fusiona |
| R: | CAGAACTTCCAGTTTGTACAGCCCCGCAAAATCATCATCAC | ||||
| pUBQ4 (K142T) | pUBQ4 | TMDTraJCDTrwB (K142T) | F: | AAGCAACACCGATGTACCCGTACCAGTGGCA | Site-Directed mutagenesisb |
| R: | GTGCCACTGGTACGGGTACATCGGTGTTGCTT | ||||
| pUBQ4GFP | pUBQ4 | TMDTraJCDTrwBGFP | F: | CCGCTCGAGATGGACGATAGAGAAAGAGG | Restriction enzymes (XhoI and BamHI) |
| R: | CGCGGATCCGATAGTCCCCTCAAC | ||||
aBerrow et al. (2007). bQuikChange II Site-Directed Mutagenesis (Stratagene, San Diego, CA, United States).