TABLE 3.
Conjugation and dominance experiments with TMDTraJCDTrwB. Transfer frequencies of plasmids pSU1456 and pKM101ΔtraJ complemented with TMDTraJCDTrwB protein have been studied.
| Plasmids in donors | T4CP | Transfer frequency |
| R388 (+) | Wild type TrwBR388 | a2.1 10–1 |
| R388ΔtrwB (pSU1456) (−) | Ø | < 10–8 |
| pKM101 (+) | Wild type TraJpKM101 | b2.2 10–1 |
| pKM101ΔtraJ (−) | Ø | < 10–8 |
| R388ΔtrwB (pSU1456) pUBQ4 | TMDTraJCDTrwB | 1.82 10–4 |
| pKM101ΔtraJ pUBQ4 | TMDTraJCDTrwB | < 10–8 |
| R388ΔtrwB (pSU1456) pUBQ4 (K142T) | TMDTraJCDTrwB (K142T) | < 10–8 |
| R388 pUBQ4 | Wild type TrwBR388 TMDTraJCDTrwB | 1.13 10–2 |
| pKM101 pUBQ4 | Wild type TraJpKM101 TMDTraJCDTrwB | 1.10 10–2 |
Additionally, the effect of the chimeric protein in the conjugative process of R388 and pKM101 has been analyzed (dominance assays, shaded in gray). E. coli DH5α and UB1637 strains were used as donor and recipient cells, respectively. Transfer frequencies were normalized to the number of transconjugants per donor and are the mean value of at least five independent experiments. (+) Positive control; (–) negative control; Ø: no T4CP. aData from Segura et al. (2013). bData from Whitaker et al. (2016).