TABLE 5.

Secondary structure components and mid-point denaturation temperatures (Tm) of TrwB_R388, TMD_TraJCD_TrwB, TrwBΔN50, TrwBΔN70, MobB_CloDF13, and MobBΔTMD.

	TrwBR388a		TMDTraJCDTrwB		TrwBΔN50a		TrwBΔN70a		MobBCloDF13		MobBΔTMD
Assignment	Position (cm–1)	Area (%)	Position (cm–1)	Area (%)	Position (cm–1)	Area (%)	Position (cm–1)	Area (%)	Position (cm–1)	Area (%)	Position (cm–1)	Area (%)
β-T + β-S	1676	5	1671	14	1676	2	1675	8	1677	7	1682	3
β-T	1661	27	/	/	1665	11	1665	11	1666	13	1665	22
α-H	1647	41	1654	35	1654	27	1653	26	1653	35	1652	29
Unordered + β-S	/	/	1640	30	1641	37	1640	39	1639	29	1639	30
β-S	1631	27	1626	17	1628	17	1627	12	/	/	/	/
Tm (°C)	48		51		48		63b		56		62

Secondary structure components were obtained from band decomposition of the amide I band of IR spectra in D₂O medium. The spectra are shown in Figure 2. Only areas above 5% are shown. For details on assignments, see the text. ^aData from Vecino et al. (2011, 2012). ^bUnpublished data from our group. β-T, β-turns; β-S, β-sheet; α-H, α-helix; Tm, mid-point denaturation temperature.