Table 1.

The extracted data and quality assessment of literature on the prognostic utility of miRNAs in osteosarcoma.

Author/miRNA	Year	Country	Sample size	Sample type	Cut off value	miRNA expression with poor prognosis	Assay method	Analysis method	Outcome	NOS score
Hong miRNA-29a/29b	2014	China	80	Serum	2.85/3.27	High	qRT-PCR	M	OS, DFS	8
Zhang miRNA-196a/196b	2014	China	100	Serum	4.86/5.48	High	qRT-PCR	M	OS, DFS	8
Cai miRNA-195	2014	China	166	Serum	1.44	Low	qRT-PCR	M	OS, DFS	8
Zhang miRNA-133b/206	2014	China	100	Serum	2.66/2.84	Low	qRT-PCR	M	OS, DFS	8
Yang miRNA-221	2015	China	108	Serum	2.42	High	qRT-PCR	M	RFS, OS	7
Wang miRNA-152	2015	China	80	Serum	NR	Low	qRT-PCR	M	OS	6
Tang miRNA-27a	2015	China	166	Serum	3.70	High	qRT-PCR	M	OS, DFS	8
Wang miRNA-191	2015	China	100	Serum	3.56	High	qRT-PCR	M	OS, DFS	7
Dong miRNA-223	2016	China	112	Serum	1.21	Low	qRT-PCR	M	OS	6
Liu miRNA-300	2016	China	114	Serum	NR	High	qRT-PCR	U/M	OS/DFS	6
Cao miRNA-326	2016	China	60	Serum	Mean level	Low	qRT-PCR	M	OS	6
Niu miRNA-95-3p	2016	China	133	Serum	0.75	Low	qRT-PCR	M	OS	7
Pang miRNA-497	2016	China	185	Serum	4.80	Low	qRT-PCR	U/M	OS	7
Li miRNA-542-3p	2017	China	76	Serum	0.87	High	qRT-PCR	U/M	OS, PFS	7
Wang miRNA-491	2017	China	102	Serum	Mean level	Low	qRT-PCR	U/M	OS	7
Wang miRNA-491-5p	2017	China	72	Serum	NR	Low	qRT-PCR	M	OS, DFS	6
Cong miRNA-124	2017	China	114	Serum	0.37-fold	Low	qRT-PCR	M	OS, DFS	6
Yao miRNA-101	2018	China	152	Serum	Median level	Low	qRT-PCR	U/M	OS, RFS	6
Zhou miRNA-139-5p	2018	China	98	Serum	Median level	Low	qRT-PCR	U/M	OS	7
Shi miRNA-194	2020	China	124	Serum	Median level	Low	qRT-PCR	M	OS,DFS	7

M, Multivariate analysis; U, Univariate analysis; DFS, Disease-free survival; OS, Overall survival; RFS, Recurrence-free survival; PFS, Progression-free survival; NR, Not reported; high, high expression; low, low expression.