FIG 3.

Increased ISVP-to-ISVP* conversion efficiency in T3D/T1L L3S2 is not due to altered interactions with viral RNA. ISVPs (2 × 10¹¹ particles/ml) derived from genome-containing or genome-deficient particles of strain T3D were divided into aliquots of equivalent volume and incubated either at 4°C or over a range of temperatures (22 to 40°C) for 20 min. The reaction mixtures were chilled on ice and digested with 0.10 mg/ml trypsin for 30 min. Following addition of loading dye, the samples were subjected to SDS-PAGE analysis. The positions of major capsid proteins are shown. μ1 runs as μ1C.