FIG 7.

T3D/T1L L3S2 affects viral replication. (A) L cell monolayers were infected with T3D or T3D/T1L L3S2 or with the indicated mutant viruses at an MOI of 0.1 PFU/cell. At 0 and 24 h postinfection, the infected cells were lysed and the viral yield was quantified by plaque assay. Error bars indicate SD. *, P < 0.05, and ***, P < 0.001, in comparison to T3D, as determined by Student's t test. Data are representative of at least 3 different experiments. (B) L cell monolayers were infected with the indicated viruses at an MOI of 10 PFU/cell. At 10 h postinfection, the cells were lysed and protein production was determined by immunoblotting. Protein quantification of 3 replicates normalized to PSTAIR is shown. Error bars indicate SD. ***, P < 0.001, in comparison to T3D, as determined by Student's t test. (C) L cell monolayers were infected with the indicated viruses at an MOI of 10 PFU/cell. The cells were lysed at 6 h postinfection, and total RNA was isolated. cDNA was generated using primers for T3D S1 and GAPDH. mRNA production was measured by RT-qPCR. Data are fold change compared to mock-infected samples and normalized to GAPDH. Error bars indicate SD. ***, P < 0.001, in comparison to T3D, as determined by Student's t test.